Probiotics for Aquaculture

Why do we need Probiotics for Aquaculture?

Probiotics for human and livestock are optimized to live in 37°C. These probiotics are inadequate to live in marine temperature(15~25°C) and salt concentration. To survive and proliferate in brine, probiotic bacteria should overcome saline condition and low temperature. Kimchi, Korean traditional vegetable food, satisfies these condition. So we separated probiotic bacteria from Kimchi.

Antibacterial activity and growth promotion in a aquacultured fish by probiotics

For the development of probiotics in aquaculture of marine organisms, three strains having psychrophilic and salt tolerant characteristics were isolated from Kimchi. Among the isolated strains, MG19, MG89 and MG208 were identified as Lactobacillus brevis, Enterococcus faecium and Lactobacillus plantarum, respectively.

The neutralized culture broth of isolated strains were tested in order to evaluate the antibacterial activity, which showed high antibacterial activity against Edwardsiella tarda, Vibrio cholerae and Pseudomonas fluorescens. In mixed culture of pathogen and isolated strain, pathogens were significantly inhibited after 2 days of cultivation but the isolated strains showed normal growth.

When the E. tarda was cultured with three isolated strains, its growth was completely inhibited after 24 hours of cultivation. The effect of isolated three strains as probiotics was investigated based on the changes in body weight of aquacultured flounder. After 50 days feeding trial, it was found that the mean body weight gain of the tested group fed by freeze dried isolated cells was significantly greater than that of the cintrol group. These results suggest that the isolated strains can play an important role as probiotics in aquaculture.

Antibacterial activity of probiotics cultivated in optimal production medium

Fig.1. Growth inhibition of Edwardsiella tarda by three probiotics(MG19, MG89, MG208) in TSB broth contained 2.5% NaCl

• ● Viable cells of E.tarda in only  E.tarda culture,
• ■ Viable cells of probiotics in mixed culture,
• ▲ Viable cells of E.tarda in mixed culture

Fig. 2. Growth inhibition of Vibrio cholerae by three probiotics(MG19, MG89, MG208) in LB broth contained 2.5% NaCl

• ● Viable cells of V.cholerae in only  V.cholerae culture,
• ■ Viable cells of  probiotics in mixed culture,
• ▲ Viable cells of V.cholerae in mixed culture

Fig. 3. Growth inhibition of Pseudomonas fluorescens by three probiotics(MG89, MG208, MG311) in LB broth

• ● Viable cells of P.fluorescens in only  P.fluorescens culture,
• ■ Viable cells of  probiotics in mixed culture,
• ▲ Viable cells of P.fluorescens in mixed culture

Effect of oral administration of probiotics on body weight gain of flounder after 50 days in feeding trials

*; mixture of MG19, MG89 and MG208

Probiotics for Aquaculture

Probiotics for domestic animal (Direct-fed Microbials)

An effective probiotic dietary supplement should

• Exert a beneficial effect on the host
• Be nonpathogenic and nontoxic
• Contain a large number of viable cells
• Be capable of surviving and metabolizing in the gut
• Remain viable during storage and use
• Be antagonistic to pathogen

Our custom probiotic formulations meet all these requirements. Growth characteristics of probiotics appear to be species specific and depend on the amount ingested and duration administered. The more the bacterial imbalance in the digestive system, the higher the dosage will be required for positive and measurable results.

Effects of Probiotics in Livestocks

• resistance to infectious disease
• improvement of digestion
• increased growth rate
• better absorption of nutrients
• provision of essential nutrients
• augmentation of milk yield
• improvement of milk quality
• increase of egg production
• reduction of bad ordor in feces

Healthier animals shall grow faster and be more productive than weak livestock. Apparently, probiotics are not an direct alternative to antibiotic treatment of acute disease. But probiotics can be used normal times to prevent infectious disease.

Probiotics for Livestock

Products for addition to high temperature treatment feed

Why do we need Probiotics for high temperature treatment feed?

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Probiotics for addition to high temperature treatment feed