Pre-implantation genetic Screening 

│ DESCRIPTION │

GENOBRO is pre-implantation genetic screening base on NGS(Next-Generation Sequencing), it is a fast and accurate screening in order to select genetically normal embryos. It has a much higher sensitivity and suspend time than conventional methods (FISH, aCGH) and can shorten inspection time.
Increased implantation and pregnancy success rates and elimination of embryos with chromosomal abnormalities can reduce abortion rate.

* Q. What is PGS?Pre-implantation genetic screening (PGS) for aneuploidy is a powerful genetic test that may be performed on embryo during IVF treatment to screen for numerical chromosomal abnormalities.
PGS is performed on a small embryo biopsy prior to transfer and identifies which embryos are chromosomally normal.  Chromosomally normal embryos are the most likely to develop to term and to be born as a healthy baby.  PGS testing helps IVF physicians and patients decide which embryos to transfer.
 
* Q.  Who should use for PGS?• Women over 35 years of age          
• Couples who have experienced several spontaneous miscarriages of unknown cause
• Couples with several cycles of IVF that have not achieved pregnancy  
• Men with low sperm concentration  
• Couples with a previous pregnancy with chromosomal abnormality
 
* Q. Key Benefit of NGS for PGS?1. Simplicity&Practical                                                              
2. Sample Throughput Flexibility and Scalability
 - PGS : Once for whole sequencing   - Sample -> Data Analysis           
3. Best Cost-Efficiency  
 - NGS enables more samples per chip(at 10 samples per 316chip)
 - No Need reference samples
4. New Diagnostic Possibilities
5. Rapid Turn Around Time  - < 24 hours workflows
 
Inspection PrincipleAfter the in vitro fertilization, the embryo is harvested from 3 to 5 days after the amplification of the DNA, and then analyzed by using the next generation genome sequencing technology, which is the latest analysis technology, to analyze the chromosome abnormality more quickly and accurately. Because of this principle, the Embryos with normal chromosomes can be screened.

* Undergoing Procedure1. Collecting a part of cells Collection 
2. DNA Separation and Amplification
3. Library production
4. Sequencing
5. Precise analysis
6. Check for chromosomal abnormality

* Proven normal Detection rate from Clinical TestAccording to the results of many overseas papers and clinical studies of famous international IVF centers, the proportion of normal embryos will decrease as the patient ages.
In the range of 35 to 40 years old, blastomere(3rd day of embryo) Stage is 20 to 30%, Blastocyst(5th day of Embryo) stage is 40 to 50%, those data are normal embryo detection rate and it is an international standard.
However, there are differences in the percentage of normal embryos depending on the individual characteristics of the patient and the status and stage of the embryo.