Saxitoxin (PSP) ELISA Test Kit
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- Required Quantity
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- Place of Origin
- Brand name
- REAGEN
- Payment Terms
- Negotiable
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- Negotiable
- Shipping / Lead Time
- Negotiable / Negotiable
- Keyword
- reagen
- Category
- Machinery & Parts
REAGEN LLC
- Country / Year Established
- U.S.A /
- Business type
- Others
- Verified Certificate
-
12
Product name | Saxitoxin (PSP) ELISA Test Kit | Certification | - |
---|---|---|---|
Category | Machinery & Parts | Ingredients | - |
Keyword | reagen | Unit Size | - |
Brand name | REAGEN | Unit Weigh | - |
origin | Stock | - | |
Supply type | - | HS code | 0103910000 |
Product Information
Saxitoxin (PSP) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Saxitoxin in mussels and water samples. Saxitoxin, the principal component of paralytic shellfish poisons (PSP), blocks the sodium ion channels in nerve and muscle membranes.
The unique features of the kit are:
§ High recovery (>80%), rapid (10 - 30 minutes), and cost-effective extraction methods
§ High sensitivity (0.02 ng/g or ppb) and low detection limit (1.2 ppb for mussel samples and 0.4 ppb for water samples).
§ A quick ELISA assay(less than 1.5 hours regardless of number of samples).
§ High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The second PSP antibody against anti-saxitoxin antibody was coated in the plate wells. During the analysis, sample is added along with the saxitoxin-horseradish peroxidase (Saxitoxin-HRP) conjugate and anti-saxitoxin antibody. If the saxitoxin residue is present in the sample, it will compete with Saxitoxin-HRP for the anti-saxitoxin antibody, thereby preventing the Saxitoxin-HRP from binding to the anti-Saxitoxin antibody. The anti-saxitoxin antibodies are then bound by the second antibody immobilized on the plate. After a washing step and addition of the TMB substrate solution, a color signal is produced. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The resulting color intensity has an inverse relationship with the saxitoxin residue concentration in the sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Saxitoxin (PSP) ELISA Test Kit has the Saxitoxin (PSP) capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
Kit Contents |
Amount |
Storage |
PSP antibody-Coated Plate |
1 x 96-well Plate(8 wells x 12 strips) |
2-8°C |
Saxitoxin (PSP) Standards: Negative control (white cap tube) 0.02 ng/mL (yellow cap tube) 0.04 ng/mL (orange cap tube) 0.08 ng/mL (pink cap tube) 0.16ng/mL (purple cap tube) 0.32ng/mL (blue cap tube) |
0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL |
2-8°C |
Anti-Saxitoxin Antibody #1 |
6 ml |
2-8°C * |
HRP-Conjugated Antibody #2 |
6 mL |
2-8°C |
20X Wash Solution ** |
28 mL |
2-8°C |
Stop Buffer ** |
14mL |
2-8°C |
TMB Substrate ** |
12 mL |
2-8°C |
10X Sample Extraction Buffer |
25 mL |
2-8°C |
l If you are not planning to use the kit for over 3 months, store Anti-Saxitoxin Antibody #1 and HRP-Conjugated Antibody #2 at -20°C or in a freezer.
B2B Trade
Price (FOB) | Negotiable | transportation | - |
---|---|---|---|
MOQ | Negotiable | Leadtime | Negotiable |
Payment Options | Negotiable | Shipping time | Negotiable |
- President
- MR DENG
- Address
- 102 Commerce Dr. Unit 8, Moorestown, NJ 08057
- Product Category
- Machinery & Parts
- Main Product
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