Soluble Epoxide Hydrolase Cell-Based Assay Kit

Soluble Epoxide Hydrolase Cell-Based Assay Kit


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U.S.A
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Keyword
assay kit
Category
Other Pharmaceutical Chemicals

Creative Biomart

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BIZ
Country / Year Established
U.S.A U.S.A / 2015
Business type
Manufacturer
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Category Other Pharmaceutical Chemicals Ingredients -
Keyword assay kit Unit Size -
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origin U.S.A Stock -
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Product Information

Mammalian soluble epoxide hydrolase (sEH) is a member of the α/β-hydrolase fold family of enzymes that catalyze the hydrolysis of exogenous and endogenous epoxides to vicinal diols. sEH is a homodimer consisting of two domains. The C-terminal domain is responsible for the epoxide hydrolase activity while the N-terminal domain has a catalytic center with phosphatase activity. Endogenous substrates for sEH include epoxyeicosatrienoic acids (EETs) which exhibit vasodilatory and anti-inflammatory activity. Inhibition of sEH in animal models was shown to effectively treat hypertension and vascular inflammation as well as related syndromes. These studies demonstrate the value for targeting sEH for development of small molecule inhibitors as therapeutics. ThesEH Cell-Based Assay Kit provides a convenient 96-well plate, fluorescence-based method for detecting epoxide hydrolase activity in whole cells. The assay utilizes Epoxy Fluor 7, a sensitive fluorescent substrate for sEH that can be used to monitor the activity of both human and murine enzymes. Hydrolysis of the substrate epoxide yields a highly fluorescent product, 6-methoxy-2-Naphthaldehyde, that can be monitored at excitation and emission wavelengths of 330 and 465 nm, respectively. 6-Methoxy-2-Naphthaldehyde is included to quantify enzyme activity and a recombinant sEH is included as a positive control. An sEH inhibitor AUDA is also included for checking specificity of the reaction. This assay parallels the Soluble Epoxide Hydrolase Inhibitor Screening Assay Kit which uses recombinant protein rather than whole cells for the assay. Together, both assays will help to identify whether or not an inhibitor/activator has a direct or indirect effect on the enzyme. 

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Creative Biomart

Country / Year Established
U.S.A U.S.A / 2015
Membership
BIZ
Business type
Manufacturer

8

DUNS

President
Linna
Address
Ramsey Road, Shirley, New York 11967, USA
Product Category
Other Health Care Products
Year Established
2015
Company introduction

Creative BioMart started from a small supplier with the development and production of recombinant protein products. After years of development and growth, we currently have over 100,000 protein products. In addition, we have also expanded a series of related products such as native proteins, cell/tissue lysates, chromatography, lectins, and detection kits.

In response to customers’ needs, Creative BioMart continuously launches popular categories such as cytokines, PROTAC targets, GPCRs, labeled proteins, full length proteins, virus-related proteins, CAR-T cell targets, biomarkers, CD antigens, etc. In addition, we offer therapeutic proteins, diagnostic proteins, and GMP proteins in accordance with the demands of industrial customers.

 

 

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