We offer this quick (10 min) HRP assay in a one-step, homogeneous, no wash assay system. This kit uses our fluorogenic Red HRP substrate to quantify peroxidase in solutions. It can be used for ELISAs, characterizing kinetics of enzyme reaction and high throughput screenings, etc. This Fluorimetric Peroxidase Assay Kit provides an optimized "mix and read" assay protocol that is compatible with HTS liquid handling instruments. It can detect as low as 10 uU/mL HRP. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540±10/590 ±10 nm (maximum Ex/Em = 540/590 nm) or an absorbance microplate reader at 576±5 nm.