β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit

β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit


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Place of Origin
U.S.A
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Keyword
assay kit
Category
Pharmaceutical Intermediates

Creative Biomart

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BIZ
Country / Year Established
U.S.A U.S.A / 2005
Business type
Manufacturer
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Product name β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit Certification -
Category Pharmaceutical Intermediates Ingredients -
Keyword assay kit Unit Size -
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origin U.S.A Stock -
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Product Information

β-Hydroxybutyrate (β-HB; 3-hydroxybutyric acid) is a "ketone body" which is produced in the liver, mainly from the oxidation of fatty acids, and is exported to peripheral tissues for use as an energy source. The term "ketone body" refers to three molecules, acetoacetate, β-HB, and acetone. β-HB and acetoacetate transport energy from the liver to the other tissues and acetone is generated by spontaneous decarboxylation of acetoacetate. The presence of ketosis may be normal or pathologic. Normally ketosis can indicate that lipid metabolism has been activated and the pathway of lipid degradation is intact. Normal ketosis is prevalent in many circumstances such as during fasting, after prolonged exercise or after a high fat diet. Pathological causes of ketosis include multiple organ failure, diabetes, childhood hypoglycemia, corticosteroid or growth hormone deficiency, intoxication with alcohol or salicylates and several inborn errors of metabolism. In acutely ill patients, these ketone bodies can accumulate in the body to cause ketoacidosis, which leads to the potentially life threatening condition known as metabolic acidosis. The presence and degree of ketosis can be determined by measuring blood levels of β-HB. Ordinarily, β-HB accounts for approximately 75% of the ketone bodies in serum. Measurement of β-HB provides a reliable index of the level of ketoacidosis, including the detection of subclinical ketosis. In diabetics, β-HB measurements (and blood glucose) can be used for the assessment of the severity of diabetic coma and is essential for the exclusion of hyperosmolar non-ketotic diabetic coma. The measurement of β-HB is also used to monitor insulin requirements, based on existing hyperketonemia. β-HB has more recently been evaluated for use in neurodegenerative diseases and inhibition of adipocyte lipolysis. Theβ-HB (Ketone Body) Assay Kit provides a simple, reproducible, and sensitive tool for measuring β-HB levels in plasma, serum, urine, cell lysates, or tissue homogenates. The method for β-HB determination is based upon the oxidation of D-3-Hydroxybutyrate to acetoacetate by the enzyme 3-hydroxybutyrate dehydrogenase.16 Concomitant with this oxidation, the cofactor NAD+ is reduced to NADH. In the presence of diaphorase, NADH reacts with the colorimetric detector WST-1 to produce a formazan dye with an absorbance maximum at 445-455 nm. The absorbance of the dye is directly proportional to the β-HB concentration. 

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Price (FOB) Negotiable transportation -
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Creative Biomart

Country / Year Established
U.S.A U.S.A / 2005
Membership
BIZ
Business type
Manufacturer

8

President
Linna
Address
45-1 Ramsey Road, Shirley, New York 11967, USA
Product Category
Other Health Care Products
Year Established
2005
No. of Total Employees
1-50
Company introduction
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Creative BioMart provides quality recombinant proteins, diagnostic antibodies and antigens, diagnostic enzymes and pharmaceutical enzymes to the research community of biology, clinical research, molecular diagnostics and biopharmaceutical drug development.

We are offering more than 1,000 recombinant proteins, peptides and antibodies. All the products are rigorously tested to meet the most demanding research needs. At the same time, lowest prices in the industry are always guaranteed.

Also, in order to leverage our core competencies and resources, Creative BioMart has formed a large number of corporate partnerships and academic collaborations for product development and distribution. We welcome potential partners and distributors to explore business relationships with Creative BioMart.

 

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