Superoxide Dismutase (SOD) Assay Kit (WST-1 method)

Superoxide Dismutase (SOD) Assay Kit (WST-1 method)


Negotiable Min Order Quantity Unit

Required Quantity
Place of Origin
U.S.A
Brand name
Elabscience
Payment Terms
Others,T/T
Production method
Negotiable
Shipping / Lead Time
Negotiable / Negotiable
Keyword
assay kit, superoxide dismutase assay kit, sod assay kit wst-1 method, sod assay
Category
Medical Test Kit

Elabscience Biotechnology,Inc.

Membership
BIZ
Country / Year Established
U.S.A U.S.A / 2011
Business type
Manufacturer
Verified Certificate

12

Product name Superoxide Dismutase (SOD) Assay Kit (WST-1 method) Certification -
Category Medical Test Kit Ingredients -
Keyword assay kit , superoxide dismutase assay kit , sod assay kit wst-1 method , sod assay Unit Size -
Brand name Elabscience Unit Weigh -
origin U.S.A Stock -
Supply type - HS code -

Product Information

 

Product name: Superoxide Dismutase (SOD) Assay Kit (WST-1 method)


Catalog number: E-BC-K020


Size: 48T/96T

Detection method: Colorimetric method

Detection instrument: Microplate reader

 

Valid period: 3 months 


Lead Time: 7~10 days 

 

 

Experimental instrument

96-well microplate, Micropipettor, 37℃ incubator, Microplate reader (450 nm)

 

Application

This kit adopts the WST-1 to measure SOD activity. It can used to measure SOD activity of serum, plasma, animal tissue, plant tissue, culture cells, whole blood, erythrocyte, etc.

 

Detection significance

Superoxide dismutase plays a crucial role in the balance between oxidation and antioxidation in body. SOD can clean superoxide anion free radicals to protect cells from damage. The assay kit adopts xanthine oxidase method (hydroxylamine method) to determinate SOD activity. This method can determinate SOD activity in serum (plasma), cerebrospinal fluid, pleural effusion, ascites, renal dialysis fluid, urine, semen, red blood cell, white blood cell, platelet, myocardial cells, tumor cell culture, kinds of animals and plant tissues and cells and subcellular levels (mitochondria and microsome), and in microbial, drugs, food, beverage, cosmetics. Hydroxylamine method not only can determinate the total SOD activity, but also Mn-SOD and Cu, Zn-SOD activity.

 

Operation steps

1.  Sample pre-treatment

(1) Serum (Plasma): Centrifuge the serum (plasma) at 3500 rpm for 10 min if it’s turbid, take the supernatant to measure. The clarified serum (plasma) was diluted into different concentrations with normal saline to do a pre-experiment.

(2)  Tissue (animal) sample: Weigh the tissue accurately. Adding 9 times of the volume of phosphate buffer: (0.1mol/L, pH 7-7.4) according to the proportion of weight (g): Volume (mL) =1:9. Homogenized mechanically with a homogenizer under ice-bath condition, then centrifuge at 2500r / min for 10 min. Take the supernatant to determine.

(3)  Tissue (plant) sample: Weigh the tissue accurately. Add 9 times of the volume of phosphate buffer: (0.1mol/L, pH7-7.4) according to the proportion of weight (g): Volume (mL) =1:9. Homogenized mechanically with a homogenizer under ice-bath condition, then centrifuge at 3500 rpm for 10 min. The supernatant was diluted into different concentrations with phosphate buffer to do a pre-experiment.

 

2.  Operation table

Components

Control well

Blank control well

Sample well

Sample blank well

Sample (μL) 

 

 

20

20

ddH2(μL) 

20

20

 

 

Enzyme working solution (μL) 

20

 

20

 

Dilution buffer (μL) 

 

20

 

20

Substrate working solution (μL) 

200

200

200

200

Mixing, incubation for 20 min at 37, and measuring spectrophotomete at a wavelength of 450 nm.

[Notices]: Set 1~2 wells of Control, Blank control, Sample blank for each experiment.


Product performance index

1.  Stability: intra-assay CV = 5.05%, inter-assay CV = 3.32%.

2.  Detection Limit: 0.5 U/mL.

3.  ODControl≥0.2

4.  Reaction temperature: 37

 

B2B Trade

Price (FOB) Negotiable transportation -
MOQ Negotiable Leadtime Negotiable
Payment Options Others,T/T Shipping time Negotiable

Elabscience Biotechnology,Inc.

Country / Year Established
U.S.A U.S.A / 2011
Membership
BIZ
Business type
Manufacturer

12

President
Yibing Leng
Address
14780 Memorial Drive, Suite 216, Houston, Texas 77079.
Product Category
Beauty & Personal Care,Industrial Supplies,Medical Test Kit
Year Established
2011
No. of Total Employees
101-500
Company introduction
<p>Elabscience specializes in immunodiagnostic technology for life science community. We have complete platform for R&D and manufacture. At the same time, we have in house QC for every product, endeavoring to keep your experiment results more consistent and precise. Through unremitting effort and development, our customers have spread in more than 100 countries all over the world.


Elabscience major products cover Proteins, Antibodies, ELlSA Kits, CLIA Kits, Labeling KitsBiochemical Kits and Other Reagents.

Elabscience also offers custom services for our customers including Protein Expression and Purification Service, Custom Antibody Service, Custom ELISA Service, Cell Biology Service, Molecular Biology Service and Whole Research Project Solutions. 

 

Factory Information

Elabscience Biotechnology,Inc.

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