Superoxide Dismutase (SOD) Assay Kit (WST-1 method)
Negotiable Min Order Quantity Unit
- Required Quantity
-
- Place of Origin
- U.S.A
- Brand name
- Elabscience
- Payment Terms
- Others,T/T
- Production method
- Negotiable
- Shipping / Lead Time
- Negotiable / Negotiable
- Category
- Medical Test Kit
Elabscience Biotechnology,Inc.
- Verified Certificate
-
12
Product name | Superoxide Dismutase (SOD) Assay Kit (WST-1 method) | Certification | - |
---|---|---|---|
Category | Medical Test Kit | Ingredients | - |
Keyword | assay kit , superoxide dismutase assay kit , sod assay kit wst-1 method , sod assay | Unit Size | - |
Brand name | Elabscience | Unit Weigh | - |
origin | U.S.A | Stock | - |
Supply type | - | HS code | - |
Product Information
Product name: Superoxide Dismutase (SOD) Assay Kit (WST-1 method)
Catalog number: E-BC-K020
Detection instrument: Microplate reader
Valid period: 3 months
Experimental instrument
96-well microplate, Micropipettor, 37℃ incubator, Microplate reader (450 nm)
Application
This kit adopts the WST-1 to measure SOD activity. It can used to measure SOD activity of serum, plasma, animal tissue, plant tissue, culture cells, whole blood, erythrocyte, etc.
Detection significance
Superoxide dismutase plays a crucial role in the balance between oxidation and antioxidation in body. SOD can clean superoxide anion free radicals to protect cells from damage. The assay kit adopts xanthine oxidase method (hydroxylamine method) to determinate SOD activity. This method can determinate SOD activity in serum (plasma), cerebrospinal fluid, pleural effusion, ascites, renal dialysis fluid, urine, semen, red blood cell, white blood cell, platelet, myocardial cells, tumor cell culture, kinds of animals and plant tissues and cells and subcellular levels (mitochondria and microsome), and in microbial, drugs, food, beverage, cosmetics. Hydroxylamine method not only can determinate the total SOD activity, but also Mn-SOD and Cu, Zn-SOD activity.
Operation steps
1. Sample pre-treatment
(1) Serum (Plasma): Centrifuge the serum (plasma) at 3500 rpm for 10 min if it’s turbid, take the supernatant to measure. The clarified serum (plasma) was diluted into different concentrations with normal saline to do a pre-experiment.
(2) Tissue (animal) sample: Weigh the tissue accurately. Adding 9 times of the volume of phosphate buffer: (0.1mol/L, pH 7-7.4) according to the proportion of weight (g): Volume (mL) =1:9. Homogenized mechanically with a homogenizer under ice-bath condition, then centrifuge at 2500r / min for 10 min. Take the supernatant to determine.
(3) Tissue (plant) sample: Weigh the tissue accurately. Add 9 times of the volume of phosphate buffer: (0.1mol/L, pH7-7.4) according to the proportion of weight (g): Volume (mL) =1:9. Homogenized mechanically with a homogenizer under ice-bath condition, then centrifuge at 3500 rpm for 10 min. The supernatant was diluted into different concentrations with phosphate buffer to do a pre-experiment.
2. Operation table
Components | Control well | Blank control well | Sample well | Sample blank well |
Sample (μL) |
|
| 20 | 20 |
ddH2O (μL) | 20 | 20 |
|
|
Enzyme working solution (μL) | 20 |
| 20 |
|
Dilution buffer (μL) |
| 20 |
| 20 |
Substrate working solution (μL) | 200 | 200 | 200 | 200 |
Mixing, incubation for 20 min at 37℃, and measuring spectrophotomete at a wavelength of 450 nm. |
[Notices]: Set 1~2 wells of Control, Blank control, Sample blank for each experiment.
Product performance index
1. Stability: intra-assay CV = 5.05%, inter-assay CV = 3.32%.
2. Detection Limit: 0.5 U/mL.
3. ODControl≥0.2
4. Reaction temperature: 37℃
B2B Trade
Price (FOB) | Negotiable | transportation | - |
---|---|---|---|
MOQ | Negotiable | Leadtime | Negotiable |
Payment Options | Others,T/T | Shipping time | Negotiable |
- President
- Yibing Leng
- Address
- 14780 Memorial Drive, Suite 216, Houston, Texas 77079.
- Product Category
- Beauty & Personal Care,Industrial Supplies,Medical Test Kit
- Year Established
- 2011
- No. of Total Employees
- 101-500
- Company introduction
-
<p>Elabscience specializes in immunodiagnostic technology for life science community. We have complete platform for R&D and manufacture. At the same time, we have in house QC for every product, endeavoring to keep your experiment results more consistent and precise. Through unremitting effort and development, our customers have spread in more than 100 countries all over the world.
Elabscience major products cover Proteins, Antibodies, ELlSA Kits, CLIA Kits, Labeling Kits,Biochemical Kits and Other Reagents.
Elabscience also offers custom services for our customers including Protein Expression and Purification Service, Custom Antibody Service, Custom ELISA Service, Cell Biology Service, Molecular Biology Service and Whole Research Project Solutions.
- Factory Information
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Elabscience Biotechnology,Inc.
- Main Product