Catalase (CAT) Assay Kit
Negotiable Min Order Quantity Unit
- Required Quantity
-
- Place of Origin
- U.S.A
- Brand name
- Elabscience
- Payment Terms
- Others,T/T
- Production method
- Negotiable
- Shipping / Lead Time
- Negotiable / Negotiable
- Keyword
- assay kit, catalase assay kit, cat assay kit, catalase assay
- Category
- Medical Test Kit
Elabscience Biotechnology,Inc.
U.S.A
- Verified Certificate
-
12
| Product name | Catalase (CAT) Assay Kit | Certification | - |
|---|---|---|---|
| Category | Medical Test Kit | Ingredients | - |
| Keyword | assay kit , catalase assay kit , cat assay kit , catalase assay | Unit Size | - |
| Brand name | Elabscience | Unit Weigh | - |
| origin | U.S.A | Stock | - |
| Supply type | - | HS code | - |
Product Information
Product name: Catalase (CAT) Assay Kit
Catalog number: E-BC-K031
Detection method: Colorimetric method
Detection instrument: Spectrophotometry (Visible range)
Valid period: 6 months
Experimental instrument
Test tube, Micropipettor, Vortex mixer, Centrifuge, Spectrophotometer (405 nm)
Application
This kit can be used to measure CAT activity in animal serum, plasma, tissue, whole blood, red blood cells, culture cells, nutrient solution and other samples.
Detection principle
The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.
Operation steps
1. Detection of CAT activity in serum(plasma)
Operation table:
| Control tube | Sample tube |
Serum (plasma) (mL) |
| 0.1 |
Reagent 1(preheated at 37℃) (mL) | 1.0 | 1.0 |
Reagent 2 (preheated at 37℃) (mL) | 0.1 | 0.1 |
Mix, react for accurately 1min at 37℃. | ||
Reagent 3 (mL) | 1.0 | 1.0 |
Reagent 4 (mL) | 0.1 | 0.1 |
Serum (plasma) (mL)* | 0.1 |
|
Note: If there are no hemolysis and obvious blood liquid, the control tube can be replaced by double-distilled water for. Control tubes only need 1-2 for each experiment.
2. Detection of CAT activity in whole blood
Preparation of 1:99 hematolysis: Dilute 50 μL whole blood to 5 mL with double-distilled water. Mix fully and stand for 10 min.
Operation table:
| Blank tube | Sample tube | Sample Control tube |
Double-distilled water (mL) | 0.05 |
| 2.1 |
1:99 hematolysis (mL) |
| 0.05 | 0.05 |
Reagent 1 (preheated at 37℃) (mL) | 1.0 | 1.0 |
|
Reagent 2 (preheated at 37℃) (mL) | 0.1 | 0.1 |
|
Mix, react for accurately 1 min at 37℃. |
| ||
Reagent 3 (mL) | 1.0 | 1.0 |
|
Mix, then measure the OD values of each tube at 405 nm wavelength (using a cuvette of 0.5 cm diameter, set to zero with double-distilled water).
3. Detection of CAT activity in tissue homogenates
Sample preparation: Weigh the tissue accurately, add normal saline to tissue sample in a weight (g): volume (mL) ratio of 1:9,
prepare 10% tissue homogenate in ice water bath. Centrifuge the tissue homogenate for 10 min at 2500~3000 rpm. Collect the
supernatant and dilute it to the optimal concentration for detection.
Operation table:
| Control tube | Sample tube |
Tissue homogenates (mL) |
| 0.05 |
Reagent 1 (preheated at 37℃) (mL) | 1.0 | 1.0 |
Reagent 2 (preheated at 37℃) (mL) | 0.1 | 0.1 |
Mix, react for 1min(60 seconds) at ℃ accurately | ||
Reagent 3 (mL) | 1.0 | 1.0 |
Reagent 4 (mL) | 0.1 | 0.1 |
Tissue homogenates (mL)* | 0.05 |
|
Mix, then measure the OD values of each tube at 405nm wavelength (using a cuvette of 0.5 cm diameter, set to zero with double-distilled water).
Note: * If there is no obvious liquid, it can be instead of double-distilled water for control tube. Control tubes only need 1-2 for each experiment.
B2B Trade
| Price (FOB) | Negotiable | transportation | - |
|---|---|---|---|
| MOQ | Negotiable | Leadtime | Negotiable |
| Payment Options | Others,T/T | Shipping time | Negotiable |
- President
- Yibing Leng
- Address
- 14780 Memorial Drive, Suite 216, Houston, Texas 77079.
- Product Category
- Beauty & Personal Care,Industrial Supplies,Medical Test Kit
- Year Established
- 2011
- No. of Total Employees
- 101-500
- Company introduction
-
<p>Elabscience specializes in immunodiagnostic technology for life science community. We have complete platform for R&D and manufacture. At the same time, we have in house QC for every product, endeavoring to keep your experiment results more consistent and precise. Through unremitting effort and development, our customers have spread in more than 100 countries all over the world.
Elabscience major products cover Proteins, Antibodies, ELlSA Kits, CLIA Kits, Labeling Kits，Biochemical Kits and Other Reagents.
Elabscience also offers custom services for our customers including Protein Expression and Purification Service, Custom Antibody Service, Custom ELISA Service, Cell Biology Service, Molecular Biology Service and Whole Research Project Solutions.
- Factory Information
-
Elabscience Biotechnology,Inc.
- Main Product
