Malondialdehyde (MDA) Assay Kit (Cell samples)

Malondialdehyde (MDA) Assay Kit (Cell samples)


Negotiable Min Order Quantity Unit

Required Quantity
Place of Origin
U.S.A
Brand name
Elabscience
Payment Terms
Others,T/T
Production method
Negotiable
Shipping / Lead Time
Negotiable / Negotiable
Keyword
assay kit, malondialdehyde assay kit, mda assay kit, mda assay cell samples
Category
Medical Test Kit

Elabscience Biotechnology,Inc.

Membership
BIZ
Country / Year Established
U.S.A U.S.A / 2011
Business type
Manufacturer
Verified Certificate

12

Product name Malondialdehyde (MDA) Assay Kit (Cell samples) Certification -
Category Medical Test Kit Ingredients -
Keyword assay kit , malondialdehyde assay kit , mda assay kit , mda assay cell samples Unit Size -
Brand name Elabscience Unit Weigh -
origin U.S.A Stock -
Supply type - HS code -

Product Information

 

Product name: Malondialdehyde (MDA) Assay Kit (Cell samples)


Catalog number: E-BC-K028


Size: 400T

Detection method: Colorimetric method

Detection instrument: Microplate reader

 

Valid period: 12 months 


Lead Time: 7~10 days 

 

 

Experimental instrument

EP tube, Micropipettor, Microplate reader (530 nm), 96-well plates, Boiling water bath (95), Vortex mixer, Centrifuge

 

Application

This kit can be used to measure the MDA content in animal culture cells and other samples.

 

Detection significance

The body produce oxygen free radicals through the enzyme system and non-enzyme system, which can attack unsaturated fatty acid on biofilm, leading to lipid peroxidation and forming lipid peroxide, such as aldehyde group(MDA), keto group, hydroxyl group, carbonyl group etc. lipid peroxide can not only transform active oxygen to active chemical, but also enhance the effect of active oxygen. Detection of the content for MDA can reflect the level of lipid peroxidation in cells and reflect level of cellular damage indirectly.

 

Detection principle

Malonyldialdehyde (MDA) in catabolite of Lipid peroxide (LPO) can condensate with thiobarbituric acid (TBA) and form red compound, which has the maximum absorbance peak at 532 nm.  

 

Operation table

 

Blank tube

Standard tube

Sample tube

Ethanol (μL)

100

 

 

10 nmol/mLstandard solution (μL)

 

100

 

Test sample (μL)

 

 

100

Working solution (μL)

1000

1000

1000

Cover the tubes, mix with vortex mixers, mix with vortex mixers and incubate for 40 min at above 95. Cool the tubes with running water. Centrifuge for 10 minutes at 4000 rpm. Measure the OD value of empty microtiter plate at 530 nm wavelength. Accurately add 0.25 mL reaction solution of each tube into a new 96-well plate, then measure the OD value of each well (minus the empty plate reader when calculating).

 

B2B Trade

Price (FOB) Negotiable transportation -
MOQ Negotiable Leadtime Negotiable
Payment Options Others,T/T Shipping time Negotiable

Elabscience Biotechnology,Inc.

Country / Year Established
U.S.A U.S.A / 2011
Membership
BIZ
Business type
Manufacturer

12

President
Yibing Leng
Address
14780 Memorial Drive, Suite 216, Houston, Texas 77079.
Product Category
Beauty & Personal Care,Industrial Supplies,Medical Test Kit
Year Established
2011
No. of Total Employees
101-500
Company introduction
<p>Elabscience specializes in immunodiagnostic technology for life science community. We have complete platform for R&D and manufacture. At the same time, we have in house QC for every product, endeavoring to keep your experiment results more consistent and precise. Through unremitting effort and development, our customers have spread in more than 100 countries all over the world.


Elabscience major products cover Proteins, Antibodies, ELlSA Kits, CLIA Kits, Labeling KitsBiochemical Kits and Other Reagents.

Elabscience also offers custom services for our customers including Protein Expression and Purification Service, Custom Antibody Service, Custom ELISA Service, Cell Biology Service, Molecular Biology Service and Whole Research Project Solutions. 

 

Factory Information

Elabscience Biotechnology,Inc.

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