Product name: IP3(Inositol Triphosphate) ELISA Kit

Catalog number: E-EL-0059

Size: 96T

Reactivity: Universal

Detection Range:   15.63~1000 pg/mL

Sensitivity: 9.38 pg/mL

Lead time: 7~10days 

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of  IP3 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with IP3. During the reaction,  IP3 in the sample or standard competes with a fixed amount of IP3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  IP3. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  IP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity

This kit recognizes some recombinant and natural IP3. No significant cross-reactivity or interference was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IP3 were tested 20 times on one plate, respectively.

Kit components & Storage

The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.