Human IL-4(Interleukin 4) ELISA Kit
Negotiable Min Order Quantity Unit
- Required Quantity
-
- Place of Origin
- U.S.A
- Brand name
- Elabscience
- Payment Terms
- Others,T/T
- Production method
- Negotiable
- Shipping / Lead Time
- Negotiable / Negotiable
- Category
- Medical Test Kit
Elabscience Biotechnology,Inc.
U.S.A
- Verified Certificate
-
12
| Product name | Human IL-4(Interleukin 4) ELISA Kit | Certification | - |
|---|---|---|---|
| Category | Medical Test Kit | Ingredients | - |
| Keyword | elisa kit , human il-4 elisa kit , il-4 elisa kit , interleukin 4 elisa kit | Unit Size | - |
| Brand name | Elabscience | Unit Weigh | - |
| origin | U.S.A | Stock | - |
| Supply type | - | HS code | - |
Product Information
Product name: Human IL-4(Interleukin 4) ELISA Kit
Catalog number: E-EL-H0101
Size: 96T
Reactivity: Human
Detection Range: 31.25~2000 pg/mL
Sensitivity: 18.75 pg/mL
Lead time: 7~10days
Intended use
This ELISA kit can be applied to the in vitro quantitative determination of Human IL-4 concentrations in serum, plasma and other biological fluids.
Test principle
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-4. The concentration of Human IL-4 in samples can be calculated by comparing the OD of the samples to the standard curve.
Specificity
This kit recognizes some recombinant and natural Human IL-4. No significant cross-reactivity or interference was observed.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high leve Human IL-4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Human IL-4 were tested on 3 different plates, 20 replicates in each plate.
Kit components & Storage
The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate(Dismountable) | 8 wells ×12 strips | -20℃, 6 months |
| Reference Standard | 2 vials | |
| Concentrated Biotinylated Detection Ab (100×) | 1 vial, 120 μL | |
| Concentrated HRP Conjugate (100×) | 1 vial, 120 μL | -20℃(shading light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 4℃, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 10 mL | |
| HRP Conjugate Diluent | 1 vial, 10 mL | |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
| Substrate Reagent | 1 vial, 10 mL | 4℃(shading light) |
| Stop Solution | 1 vial, 10 mL | 4℃ |
| Plate Sealer | 5 pieces | |
| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.
Citations
1.Publication: Li W, Dong H, Huang Y, et al. Clonorchis Sinensis Co-Infection Could Affect The Disease State And Treatment Response Of Hbv Patients[J]. Plos Neglected Tropical Diseases, 2016.
Species: Human
Sample Type: culture supernatants and serum
2.Publication: Li H T, Zhang T T, Chen Z G, et al. Intranasal Administration Of CpG Oligodeoxynucleotides Reduces Lower Airway Inflammation In A Murine Model Of Combined Allergic Rhinitis And Asthma Syndrome[J]. International Immunopharmacology, 2015, 28: 390-398.
Species: Human
Sample Type: bronchoalveolar lavage fluid
3.Publication: Wang Z Y, Jiang M J, Wu M H, et al. Inflammatory Characteristics In Different Types Of Nonallergic Rhinitis[J]. International Journal of Clinical and Experimental Pathology, 2017, 10(3): 2887-2894.
Species: Human
Sample Type: culture supernatant
4.Publication: Xia G, Xin S, Zheng X, et al. Decreased Expression Of Programmed Death 1 On Peripheral Blood Lymphocytes Disrupts Immune Homeostasis In Peripartum Cardiomyopathy[J]. International Journal of Cardiology, 2016, 223: 842-847.
Species: Human
Sample Type: serum
5.Publication: Xia G, Zheng X, Yao X, et al. Expression Of Programmed Cell Death-1 And Its Ligand B7 Homolog 1 In Peripheral Blood Lymphocytes From Patients With Peripartum Cardiomyopathy[J]. Clinical Cardiology, 2016.
Species: Human
Sample Type: serum
6.Publication: Fan H, Li L, Zhang Y, et al. Pkcζ In Prostate Cancer Cells Represses The Recruitment And M2 Polarization Of Macrophages In The Prostate Cancer Microenvironment[J]. Tumor Biology, 2017.
Species: Human
Sample Type: cell lysis and culture supernatant
B2B Trade
| Price (FOB) | Negotiable | transportation | - |
|---|---|---|---|
| MOQ | Negotiable | Leadtime | Negotiable |
| Payment Options | Others,T/T | Shipping time | Negotiable |
- President
- Yibing Leng
- Address
- 14780 Memorial Drive, Suite 216, Houston, Texas 77079.
- Product Category
- Beauty & Personal Care,Industrial Supplies,Medical Test Kit
- Year Established
- 2011
- No. of Total Employees
- 101-500
- Company introduction
-
<p>Elabscience specializes in immunodiagnostic technology for life science community. We have complete platform for R&D and manufacture. At the same time, we have in house QC for every product, endeavoring to keep your experiment results more consistent and precise. Through unremitting effort and development, our customers have spread in more than 100 countries all over the world.
Elabscience major products cover Proteins, Antibodies, ELlSA Kits, CLIA Kits, Labeling Kits，Biochemical Kits and Other Reagents.
Elabscience also offers custom services for our customers including Protein Expression and Purification Service, Custom Antibody Service, Custom ELISA Service, Cell Biology Service, Molecular Biology Service and Whole Research Project Solutions.
- Factory Information
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Elabscience Biotechnology,Inc.
- Main Product
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