Human IL-4(Interleukin 4) ELISA Kit

Human IL-4(Interleukin 4) ELISA Kit


Negotiable Min Order Quantity Unit

Required Quantity
Place of Origin
U.S.A
Brand name
Elabscience
Payment Terms
Others,T/T
Production method
Negotiable
Shipping / Lead Time
Negotiable / Negotiable
Keyword
elisa kit, human il-4 elisa kit, il-4 elisa kit, interleukin 4 elisa kit
Category
Medical Test Kit

Elabscience Biotechnology,Inc.

Membership
BIZ
Country / Year Established
U.S.A U.S.A / 2011
Business type
Manufacturer
Verified Certificate

12

Product name Human IL-4(Interleukin 4) ELISA Kit Certification -
Category Medical Test Kit Ingredients -
Keyword elisa kit , human il-4 elisa kit , il-4 elisa kit , interleukin 4 elisa kit Unit Size -
Brand name Elabscience Unit Weigh -
origin U.S.A Stock -
Supply type - HS code -

Product Information

 

Product name: Human IL-4(Interleukin 4) ELISA Kit

 

Catalog number: E-EL-H0101

 

Size: 96T

Reactivity: Human

 

Detection Range:  31.25~2000 pg/mL

 

Sensitivity: 18.75 pg/mL

 

Lead time: 7~10days 

 

 

Intended use

This ELISA kit can be applied to the in vitro quantitative determination of Human IL-4 concentrations in serum, plasma and other biological fluids.

 

Test principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-4. The concentration of Human IL-4 in samples can be calculated by comparing the OD of the samples to the standard curve.

 

Specificity

This kit recognizes some recombinant and natural Human IL-4. No significant cross-reactivity or interference was observed.

 

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high leve Human IL-4 were tested 20 times on one plate, respectively. 
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Human IL-4 were tested on 3 different plates, 20 replicates in each plate.

Kit components & Storage

The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells ×12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL4℃, 6 months
Biotinylated Detection Ab Diluent1 vial, 10 mL
HRP Conjugate Diluent1 vial, 10 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL4℃(shading light)
Stop Solution1 vial, 10 mL4℃
Plate Sealer5 pieces 
Product Description1 copy
Certificate of Analysis1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.

 

Citations

 

1.Publication: Li W, Dong H, Huang Y, et al. Clonorchis Sinensis Co-Infection Could Affect The Disease State And Treatment Response Of Hbv Patients[J]. Plos Neglected Tropical Diseases, 2016.
Species: Human
Sample Type: culture supernatants and serum

2.Publication: Li H T, Zhang T T, Chen Z G, et al. Intranasal Administration Of CpG Oligodeoxynucleotides Reduces Lower Airway Inflammation In A Murine Model Of Combined Allergic Rhinitis And Asthma Syndrome[J]. International Immunopharmacology, 2015, 28: 390-398.
Species: Human
Sample Type: bronchoalveolar lavage fluid

3.Publication: Wang Z Y, Jiang M J, Wu M H, et al. Inflammatory Characteristics In Different Types Of Nonallergic Rhinitis[J]. International Journal of Clinical and Experimental Pathology, 2017, 10(3): 2887-2894.
Species: Human
Sample Type: culture supernatant

4.Publication: Xia G, Xin S, Zheng X, et al. Decreased Expression Of Programmed Death 1 On Peripheral Blood Lymphocytes Disrupts Immune Homeostasis In Peripartum Cardiomyopathy[J]. International Journal of Cardiology, 2016, 223: 842-847.
Species: Human
Sample Type: serum

5.Publication: Xia G, Zheng X, Yao X, et al. Expression Of Programmed Cell Death-1 And Its Ligand B7 Homolog 1 In Peripheral Blood Lymphocytes From Patients With Peripartum Cardiomyopathy[J]. Clinical Cardiology, 2016.
Species: Human
Sample Type: serum

6.Publication: Fan H, Li L, Zhang Y, et al. Pkcζ In Prostate Cancer Cells Represses The Recruitment And M2 Polarization Of Macrophages In The Prostate Cancer Microenvironment[J]. Tumor Biology, 2017.
Species: Human
Sample Type: cell lysis and culture supernatant

 

B2B Trade

Price (FOB) Negotiable transportation -
MOQ Negotiable Leadtime Negotiable
Payment Options Others,T/T Shipping time Negotiable

Elabscience Biotechnology,Inc.

Country / Year Established
U.S.A U.S.A / 2011
Membership
BIZ
Business type
Manufacturer

12

President
Yibing Leng
Address
14780 Memorial Drive, Suite 216, Houston, Texas 77079.
Product Category
Beauty & Personal Care,Industrial Supplies,Medical Test Kit
Year Established
2011
No. of Total Employees
101-500
Company introduction
<p>Elabscience specializes in immunodiagnostic technology for life science community. We have complete platform for R&D and manufacture. At the same time, we have in house QC for every product, endeavoring to keep your experiment results more consistent and precise. Through unremitting effort and development, our customers have spread in more than 100 countries all over the world.


Elabscience major products cover Proteins, Antibodies, ELlSA Kits, CLIA Kits, Labeling KitsBiochemical Kits and Other Reagents.

Elabscience also offers custom services for our customers including Protein Expression and Purification Service, Custom Antibody Service, Custom ELISA Service, Cell Biology Service, Molecular Biology Service and Whole Research Project Solutions. 

 

Factory Information

Elabscience Biotechnology,Inc.

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