FAQ-Post Translational Modification
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- U.S.A
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- Category
- Pharmaceutical Intermediates
Creative Proteomics
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Product Information
- What are the concerns about mapping a phosphorylation site in my protein?
You will have two major concerns:
Coverage: Can the mass spectrometry technique(s) deliver high sequence coverage? With higher coverage, there is a greater statistical chance to detect and identify the peptide containing a modification group.
Occupancy of the modification site: What percent of the protein preparation is modified? If it is low, then the chance of detecting the modified peptide decreases. If the occupancy is high (>30%), then this works in the favor of identifying a modification site. - How can coverage be increased for mapping phosphorylation or methylation sites?
First, note that, due to its unique sequences, every protein has a different potential to be mapped at high sequence coverage.
Coverage for all proteins increases by:
a) Starting with a higher amount of protein (>5 μg of protein is desirable) for MS analysis
b) Making sure that the protein is pure
c) Using multiple MS instruments/techniques for analysis will increase coverage
d) Enriching the phosphopeptides using a TiO2 column
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- President
- Dr.Chen
- Address
- 45-1 Ramsey Road
- Product Category
- Other Pharmaceutical Chemicals
- Year Established
- 2008
- No. of Total Employees
- 101-500
- Company introduction
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Creative Proteomics is the proteomics division of CD Inc, an integrated CRO company that provides a full range of drug development services, including Molecular Biology, Biochemistry, Systems Biology, Organic Chemistry, Genomics, Bioinformatics, Structural Biology, Preclinical and Clinical studies.
Creative Proteomics specializes in a full range of services to support various proteome-related researches from identification of single proteins to large-scale proteomic studies. We have one of the most advanced proteomics platforms in the world and our staff scientists are experienced proteomics professionals. Our proteome analysis platform provides protein separation, characterization, identification and quantification services, featured with high throughput and super-sensitivity. In addition, analyses of protein post-translational modifications such as phosphorylation and glycosylation are available.
- Main Markets
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U.S.A
- Main Product
