1D SDS-PAGE,IEF

1D SDS-PAGE,IEF


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Place of Origin
U.S.A
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Keyword
1d, sds-page
Category
Organic Intermediate

Creative Proteomics

Membership
BIZ
Country / Year Established
U.S.A U.S.A / 2008
Business type
Manufacturer
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9

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Product Information

 

Polyacrylamide gel electrophoresis (PAGE) is one of the most frequently employed techniques for separating macromolecules including DNA, RNA, and proteins

Electrophoresis is in general the process of applying an electric field to move charged molecules through a solution. In this technique, the mobility of a charged molecule is directly proportional to its net charge and the resistance of the solution through which it is moving.

Sodium Dodecyl Sulfate (SDS) is an anionic detergent, and its molecules have a net negative charge within a wide pH range when dissolved. A polypeptide chain binds certain amount of SDS in proportion to its relative molecular mass, and the negative charges on SDS destroy most of the complex structure of proteins, especially after previous reduction with reagents such as DTT. Then the protein becomes a rod-like, negatively charge macromolecule with comparable hydrodynamic qualities, the mobility of which depends only on their molecular mass.

However, when a mixture of proteins is added onto the gel, the smallest of the proteins will start to make their way through the gel even before the entire sample has entered the gel. In order to achieve better separation, the entire sample is condensed into the narrowest possible band right at the interface of the gel through a neat trick called a stacking gel, and here is how it works.

 

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Creative Proteomics

Country / Year Established
U.S.A U.S.A / 2008
Membership
BIZ
Business type
Manufacturer

9

President
Dr.Chen
Address
45-1 Ramsey Road
Product Category
Other Pharmaceutical Chemicals
Year Established
2008
No. of Total Employees
101-500
Company introduction
<p> 

Creative Proteomics is the proteomics division of CD Inc, an integrated CRO company that provides a full range of drug development services, including Molecular Biology, Biochemistry, Systems Biology, Organic Chemistry, Genomics, Bioinformatics, Structural Biology, Preclinical and Clinical studies.

Creative Proteomics specializes in a full range of services to support various proteome-related researches from identification of single proteins to large-scale proteomic studies. We have one of the most advanced proteomics platforms in the world and our staff scientists are experienced proteomics professionals. Our proteome analysis platform provides protein separation, characterization, identification and quantification services, featured with high throughput and super-sensitivity. In addition, analyses of protein post-translational modifications such as phosphorylation and glycosylation are available.

 

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