Our company         Elabscience Biotechnology Co., Ltd.

Product name         Human RARα (Retinoic Acid Receptor Alpha) ELISA Kit

Catalog No.             E-EL-H2411

Package                  96T/kit

Detection Range     0.156-10ng/mL

Sensitivity              0.094 ng/mL

Specificity               This kit recognizes natural and recombinant Human RARα. No significant cross-reactivity or interference  between Human RARα and analogues was observed. Note： Limited by existing techniques, cross-reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between Human RARα and all the analogues.

Repeatability           Coefficient of variation were<10%.

Test principle           This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RARα.Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human RARα and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RARα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RARα. You can calculate the concentration of Human RARα in the samples by comparing the OD of the samples to the standard curve.