Mouse IL-12 Elisa kit

Catalog No: E-EL-M0726

Elabscience Biotechnology Co., Ltd.

Package: 96T/kit

Intended use

This ELISA kit applies to the in vitro quantitative determination of Mouse IL-12 concentrations in serum, plasma and other biological fluids.

Detection Range

15.63 to 1000 pg/mL.

Sensitivity

The minimum detectable dose of Mouse IL-12 is 9.38 pg/mL.

Specificity

This kit recognizes natural and recombinant Mouse IL-12. No significant cross reactivity or interference between Mouse IL-12 and analogues was observed.

Repeatability  

Coefficient of variation were<10%.

Test principle

This ELISA kit uses Sandwich ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to IL-12. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-12 and Avidin Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-12, biotinylated detection antibody and Avidin HRP conjugate will appear blue in color. The enzyme substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-12. You can calculate the concentration of IL-12 in the samples by comparing the O.D. of the samples to the standard curve.