Mouse TNF-α Elisa kit

Catalog No: E-EL-M0049

Elabscience Biotechnology Co., Ltd.

Package: 96T/kit

Intended use

This ELISA kit applies to the in vitro quantitative determination of Mouse TNF-α concentrations in serum, plasma and other biological fluids.

Detection Range

31.25 to 2000 pg/mL.

Sensitivity

The minimum detectable dose of Mouse TNF-α is 18.75 pg/mL.

Specificity

This kit recognizes natural and recombinant Mouse TNF-α. No significant cross reactivity or interference between Mouse TNF-α and analogues was observed.

Repeatability  

Coefficient of variation were<10%.

Test principle

This ELISA kit uses Sandwich ELISA as the method. The micro ELISA plate provided in this kit has been precoated with an antibody specific to TNF-α. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TNF-α and Avidin Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNF-α, biotinylated detection antibody and Avidin HRP conjugate will appear blue in color. The enzyme substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TNF-α. You can calculate the concentration of TNF-α in the samples by comparing the O.D. of the samples to the standard curve.